The aim of this thesis work was to evaluate the efficacy of two strategies to load drugs into gelatin hydrogels. Drug was added by interpenetration during gelatin hydrogel synthesis or by absorption. The so obtained hydrogels were physico-chemically and mechanically characterized. The hydrogels demonstrated to be stable in water at 37°C up to three weeks with high water uptake. They also showed competitive mechanical properties, tunable with the degree of gelatin crosslinking. The drug loading/release kinetics was investigated and the results exhibited a uniform release within 3 days with no burst release. The released drug was proven to be active by in vitro biological tests. L929 fibroblasts were seeded on the hydrogels and their viability was proven to be comparable to the negative control. All developed drug loaded hydrogels resulted a suitable and promising material for wound/burn healing systems as well as tissue engineering scaffold matrixes.
Lo scopo del presente lavoro di tesi è stato quello di valutare l’efficienza di due strategie di caricamento di eparina in idrogeli di gelatina reticolati. L’eparina è stata aggiunta agli idrogeli durante la sintesi o per assorbimento. Sono state investigate le proprietà chimico-fisiche e meccaniche degli idrogeli così ottenuti. Gli idrogeli si sono dimostrati stabili in acqua a 37°C fino a tre settimane con alti valori di rigonfiamento. Hanno mostrato proprietà meccaniche ingegnerizzabili modificando il grado di reticolazione della gelatina. Le prove di rilascio hanno mostrato un rilascio uniforme fino a 3 giorni. Inoltre, prove di citocompatibilità in vitro con fibroblasti murini L929 hanno dimostrato una buona compatibilità cellulare fino a 7 giorni dalla semina. Nel complesso gli idrogeli sviluppati nel presente lavoro di tesi sono risultati promettenti per la progettazione di materiali per la guarigione di ferite e ustioni, oltre che per l’uso come matrici di scaffold per l’ingegneria dei tessuti.
Crosslinked gelatin hydrogels as heparin controlled delivery systems
GRITSCH, LUKAS;MOTTA, FEDERICO LEONARDO
2013/2014
Abstract
The aim of this thesis work was to evaluate the efficacy of two strategies to load drugs into gelatin hydrogels. Drug was added by interpenetration during gelatin hydrogel synthesis or by absorption. The so obtained hydrogels were physico-chemically and mechanically characterized. The hydrogels demonstrated to be stable in water at 37°C up to three weeks with high water uptake. They also showed competitive mechanical properties, tunable with the degree of gelatin crosslinking. The drug loading/release kinetics was investigated and the results exhibited a uniform release within 3 days with no burst release. The released drug was proven to be active by in vitro biological tests. L929 fibroblasts were seeded on the hydrogels and their viability was proven to be comparable to the negative control. All developed drug loaded hydrogels resulted a suitable and promising material for wound/burn healing systems as well as tissue engineering scaffold matrixes.File | Dimensione | Formato | |
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2014_04_Gritsch_Motta.pdf
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https://hdl.handle.net/10589/106909