The aim of this thesis was to validate long-term and parallel neuroelectrophysio-logical recordings on an environmental controlled chamber based on Multi-electrode arrays (MEA). This system demonstrated to have potential for being a powerful instrument for the study of in vitro neuron networks, allowing uninterruptedly recordings of hippocampal neurons and in the future would be useful to investigate the differentiation of stem cells into neurons and ultimately to help understand and model disease mechanisms.
Lo scopo di questa tesi è stato quello di convalidare registrazioni neuro-elettrofisiologiche a lungo termine, all'interno di una camera di controllo ambientale basata sulla tecnologia Multielectrode arrays (MEA). Questo sistema consente di registrare ininterrottamente l’attività elettrica di neuroni ippocampali, dimostrando di poter diventare uno strumento per lo studio delle rete neuronali in vitro. In futuro potrebbe essere utile per investigare il differenziamento di cellule staminali in neuroni e per capire meccanismi molecolari di malattia.
Validation and testing of a culture chamber for long-term neurophysiological recordings with multielectrode arrays
SOUZA de OLIVEIRA, DANIELA
2017/2018
Abstract
The aim of this thesis was to validate long-term and parallel neuroelectrophysio-logical recordings on an environmental controlled chamber based on Multi-electrode arrays (MEA). This system demonstrated to have potential for being a powerful instrument for the study of in vitro neuron networks, allowing uninterruptedly recordings of hippocampal neurons and in the future would be useful to investigate the differentiation of stem cells into neurons and ultimately to help understand and model disease mechanisms.File | Dimensione | Formato | |
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2018_12_Oliveira.pdf
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https://hdl.handle.net/10589/145211