The aim of the present work was to design a microfluidic device able to generate polyplexes with different N/P ratios at the same time. After the designing, the device has been validated in all its part and, lastly, the efficiency of polyplexes produced with the device was tested on L929 cells, comparing it with that of polyplexes produced following a bulk preparation method.
Il presente lavoro ha avuto come obiettivo quello di progettare un dispositivo microfluidico in grado di generare poliplessi a diversi rapporti N/P contemporaneamente. Il dispositivo è stato successivamente validato in tutte le sue parti e, in ultimo, l'efficacia dei poliplessi da esso prodotti è stata testata su cellule L929, comparandola con quella di poliplessi prodotti utilizzando un metodo di preparazione manuale.
Design and validation of a high-throughput microfluidic device for non-viral gene
D'ALESSANDRO, FABIO
2018/2019
Abstract
The aim of the present work was to design a microfluidic device able to generate polyplexes with different N/P ratios at the same time. After the designing, the device has been validated in all its part and, lastly, the efficiency of polyplexes produced with the device was tested on L929 cells, comparing it with that of polyplexes produced following a bulk preparation method.| File | Dimensione | Formato | |
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Tesi Fabio D'Alessandro 2018 2019.pdf
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Descrizione: Testo della tesi
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https://hdl.handle.net/10589/150082