Infections related to biomaterial implantation are a major clinical problem, mainly owing to the formation of biofilm. Biofilm-associated bacteria show a decreased susceptibility to antibiotics, disinfectants and clearance by host defence. It is thus necessary to prevent bacterial colonization. For this purpose, the research is developing alternative approach to the use of antibiotics, which are based on the modification of the implant surface with the aim to inhibit bacterial adhesion and limit bacterial proliferation. One of the most promising strategies is the creation of antibacterial coatings, incorporating metallic antibacterial agents, such as Gallium. They possess many advantages like antibacterial ability, excellent biocompatibility and satisfactory stability. In this study, gallium doped zirconia coatings were developed with the aims of providing antibacterial activity. The coatings have been synthesized starting from the sol-gel technique, without the addition of surfactant molecules in the solution, which should be removed later with high-temperature thermal treatments that could cause a decline in the mechanical properties of zirconia, and applied by dip-coating technique. This coating method has advantages such as lower processing temperature, reduced film thickness, ability to form a uniform coating over complex geometric shapes, low cost and relative ease of production. Three kind of coatings were produced in this study, that differ for gallium concentration equal to the 5%, 10% and 20% of the number of zirconia moles. Several analysis were performed in order to characterize the structure of the coatings. Moreover, in vitro tests were performed to evaluate the cytocompatibility and antibacterial activity of the coatings.
Le infezioni legate all'impianto di biomateriali sono un grave problema clinico, principalmente a causa della formazione di biofilm. I batteri associati ai biofilm mostrano una minore suscettibilità ad antibiotici, disinfettanti e al sistema immunitario dell’organismo ospite. È quindi necessario prevenire la colonizzazione batterica. A tale scopo, la ricerca sta sviluppando un approccio alternativo all'uso di antibiotici, che si basano sulla modifica della superficie dell'impianto con l'obiettivo di inibire l'adesione batterica e limitare la proliferazione batterica. Una delle strategie più promettenti è la creazione di rivestimenti antibatterici, che incorporano agenti antibatterici metallici, come il Gallio. Possiedono molti vantaggi come la capacità antibatterica, l'eccellente biocompatibilità e una stabilità soddisfacente. In questo studio, sono stati sviluppati rivestimenti in zirconia dopata con gallio con l'obiettivo di fornire un’attività antibatterica. I rivestimenti sono stati sintetizzati a partire dalla tecnica sol-gel, senza l’aggiunta in soluzione di molecole di surfactante, le quali andrebbero rimosse in seguito con trattamenti termici ad alta temperatura che potrebbero causare un calo delle proprietà meccaniche della zirconia, e applicati con tecnica dip-coating. Questo metodo di rivestimento presenta dei vantaggi come una bassa temperatura di lavorazione, uno spessore ridotto del rivestimento, la capacità di formare un rivestimento uniforme su forme geometriche complesse, basso costo e relativa facilità di produzione. In questo studio sono stati prodotti tre tipi di rivestimenti, che differiscono per la concentrazione di gallio, pari al 5%, 10% e 20% del numero di moli di zirconia. Sono state eseguite diverse analisi per caratterizzare la struttura dei rivestimenti. Inoltre, sono stati eseguiti test in vitro per valutare la citocompatibilità e l'attività antibatterica dei rivestimenti.
Sviluppo e caratterizzazione di un rivestimento antibatterico in zirconia per impianti dentali
BURATTI, STEFANO
2018/2019
Abstract
Infections related to biomaterial implantation are a major clinical problem, mainly owing to the formation of biofilm. Biofilm-associated bacteria show a decreased susceptibility to antibiotics, disinfectants and clearance by host defence. It is thus necessary to prevent bacterial colonization. For this purpose, the research is developing alternative approach to the use of antibiotics, which are based on the modification of the implant surface with the aim to inhibit bacterial adhesion and limit bacterial proliferation. One of the most promising strategies is the creation of antibacterial coatings, incorporating metallic antibacterial agents, such as Gallium. They possess many advantages like antibacterial ability, excellent biocompatibility and satisfactory stability. In this study, gallium doped zirconia coatings were developed with the aims of providing antibacterial activity. The coatings have been synthesized starting from the sol-gel technique, without the addition of surfactant molecules in the solution, which should be removed later with high-temperature thermal treatments that could cause a decline in the mechanical properties of zirconia, and applied by dip-coating technique. This coating method has advantages such as lower processing temperature, reduced film thickness, ability to form a uniform coating over complex geometric shapes, low cost and relative ease of production. Three kind of coatings were produced in this study, that differ for gallium concentration equal to the 5%, 10% and 20% of the number of zirconia moles. Several analysis were performed in order to characterize the structure of the coatings. Moreover, in vitro tests were performed to evaluate the cytocompatibility and antibacterial activity of the coatings.File | Dimensione | Formato | |
---|---|---|---|
2019_12_Buratti.pdf
non accessibile
Descrizione: Testo della tesi
Dimensione
2.51 MB
Formato
Adobe PDF
|
2.51 MB | Adobe PDF | Visualizza/Apri |
I documenti in POLITesi sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/10589/151123