The aim of this thesis is to fabricate micron-scale cell-instructive biomaterials, so called as micro-objects, and use them as building blocks for creating 3D micro-tissues via assembling with human mesenchymal stromal cells (hMSCs). Tailoring the chemical composition, outer shape or surface properties of the micro-object enables to instruct cells, for example to differentiate them toward bone or cartilage lineage. These 3D tissue modules could be fused together to form a larger construct, resembling natural tissues. In this study, we aimed to adopt a fabrication method based on PRINT® technology, to produce large quantities of micro-objects with defined shape using biomaterials that are commonly used for bone and cartilage regeneration, such as poly (lactic acid) (PLA) and hydroxyapatite (HA).
Lo scopo di questa tesi è di fabbricare biomateriali cellule-istruttivi in scala micrometrica, chiamati micro-oggetti, e usarli come building blocks per creare micro-tessuti attraverso l’assemblamento con cellule stromali mesenchimali umane (hMSCs). Adattare la composizione chimica, la forma esterna o le proprietà di superficie dei micro-oggetti, permette di istruire le cellule, per esempio a differenziare verso lignaggio osseo o cartilagineo. Questi moduli 3D di tessuto possono essere fusi insieme in un costrutto più grande che mimi il tessuto naturale. In questo studio abbiamo adottato un metodo di fabbricazione basato sulla tecnologia PRINT®, per produrre grandi quantità di micro-oggetti con forma definita utilizzando biomateriali che sono comunemente usati per la rigenerazione dell’osso e della cartilagine, come l’acido polilattico (PLA) e l’idrossiapatite (HA).
Cell-instructive micro-objects for bottom-up regeneration of skeletal system
RUGGIERI, ILARIA
2019/2020
Abstract
The aim of this thesis is to fabricate micron-scale cell-instructive biomaterials, so called as micro-objects, and use them as building blocks for creating 3D micro-tissues via assembling with human mesenchymal stromal cells (hMSCs). Tailoring the chemical composition, outer shape or surface properties of the micro-object enables to instruct cells, for example to differentiate them toward bone or cartilage lineage. These 3D tissue modules could be fused together to form a larger construct, resembling natural tissues. In this study, we aimed to adopt a fabrication method based on PRINT® technology, to produce large quantities of micro-objects with defined shape using biomaterials that are commonly used for bone and cartilage regeneration, such as poly (lactic acid) (PLA) and hydroxyapatite (HA).File | Dimensione | Formato | |
---|---|---|---|
ILARIA RUGGIERI - Cell-instructive micro-objects for bottom up regeneration of skeletal system.pdf
non accessibile
Descrizione: Testo della tesi
Dimensione
4 MB
Formato
Adobe PDF
|
4 MB | Adobe PDF | Visualizza/Apri |
I documenti in POLITesi sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/10589/154625