The state of the current surgical techniques for oesophageal replacement underlines the need to search for new strategies. Tissue engineering is a promising candidate able to overcome all the present limitations. Cells, bioreactors and scaffolds are the three pillars on which tissue engineering is based. The aim of tis thesis was to generate suitable scaffolds for oesophageal tissue engineering by mean of decellularisation of 3, 10 and 25 kg pigs oesophagi. The starting point was the protocol and the bioreactor for rat oesophagus decellularisation, it was required an upscaling and optimisation in order to obtain a versatile system adaptable to multiple pig oesophagus sizes. Complete decellularisation of every oesophagus size was obtained with the combination of the updated protocol and a novel 3D printed set of connectors. Whole bioreactor 3D printing and possible future developments are also explored.
Lo stato delle attuali tecniche chirurgiche per il rimpiazzo esofageo sottolinea la necessità di ricercare nuove strategie. L'ingegneria dei tessuti è un candidato promettente in grado di superare tutti i limiti attuali. Cellule, bioreattori e scaffold sono i tre pilastri su cui si basa questa scienza. Lo scopo di questa tesi era quello di generare scaffold idonei per l'ingegnerizzazione del tessuto esofageo mediante decellularizzazione di esofagi di maiali da 3, 10 e 25 kg. Il punto di partenza erano Il protocollo e il bioreattore per la decellularizzazione dell'esofago di ratto, era richiesta quindi un’ottimizzazione per ottenere un sistema versatile e adattabile a diverse dimensioni di esofago di maiale. La decellularizzazione completa di tutte le taglie di esofagi è stata raggiunta con la combinazione del protocollo aggiornato e un nuovo set di connettori stampati in 3D. Vengono anche esplorate la stampa 3D dell'intero bioreattore e possibili sviluppi futuri.
Oesophageal tissue engineering : upscaling of the decellularised biological scaffold
Lutman, Roberto
2019/2020
Abstract
The state of the current surgical techniques for oesophageal replacement underlines the need to search for new strategies. Tissue engineering is a promising candidate able to overcome all the present limitations. Cells, bioreactors and scaffolds are the three pillars on which tissue engineering is based. The aim of tis thesis was to generate suitable scaffolds for oesophageal tissue engineering by mean of decellularisation of 3, 10 and 25 kg pigs oesophagi. The starting point was the protocol and the bioreactor for rat oesophagus decellularisation, it was required an upscaling and optimisation in order to obtain a versatile system adaptable to multiple pig oesophagus sizes. Complete decellularisation of every oesophagus size was obtained with the combination of the updated protocol and a novel 3D printed set of connectors. Whole bioreactor 3D printing and possible future developments are also explored.File | Dimensione | Formato | |
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2021_04_Lutman.pdf
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Descrizione: Testo della tesi
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https://hdl.handle.net/10589/173508