This thesis project has been conducted at CRI (Cambridge Raman Imaging), a spin-off company of Politecnico di Milano and Universisty of Cambridge, whose main aim is developing a compact, coherent Raman imaging platform for in-vitro diagnostics. In particular, the start-up is specialized in stimulated Raman analysis of tissues: the idea is that the stimulated Raman spectrum can be used as a bio-marker to identify specific diseases or disorders, thus enabling the discrimination between healthy cells/tissues and unhealthy ones. This project consisted in the development, characterisation and test of a custom Stimulated Raman Scattering (SRS) microscope from scratch; this means the apparatus should be flexible, suitable for analysis of different samples (not only biological ones) and capable to encompass different optical microscopy techniques. For the realization of the system, simple optical components were adopted, without integrated devices produced by third parts. Essentiality of the setup is what makes it complex, since it is more critical. In order to build the custom microscope, I worked with a PhD student, Matteo Corti, and I was supervised by the R&D Manager of the company, Francesco Crisafi. This work belongs to CRIMSON project: CRIMSON aims to provide a next-generation bio-photonics imaging device based on vibrational spectroscopy, with the potential to revolutionize the study of the cellular origin of diseases allowing for novel approaches towards personalized therapy.
Questo progetto di tesi è stato condotto presso CRI (Cambridge Raman Imaging), un’azienda spin-off del Politecnico di Milano e dell’Università di Cambridge, il cui obiettivo principale è lo sviluppo di una piattaforma compatta per l’imaging Raman coerente, destinata alla diagnostica in vitro. In particolare, la start-up è specializzata nell’analisi Raman stimolata di tessuti biologici: l’idea alla base è che lo spettro Raman stimolato possa essere utilizzato come biomarcatore per identificare specifiche malattie o disturbi, permettendo così la distinzione tra cellule/tessuti sani e malati. Questo progetto tratta circa lo sviluppo, la caratterizzazione ed i test di un microscopio SRS custom, realizzato da zero; ciò serve a rendere flessibile, adatto all’analisi di diversi campioni, come le microplastiche, e in grado di sfruttare differenti tecniche di microscopia ottica. Per la realizzazione del sistema, sono state utilizzate componenti ottiche semplici, senza dispositivi integrati prodotti da terze parti. L’essenzialità del setup ne determina anche la complessità, poiché richiede un livello di precisione maggiore. Per la costruzione del microscopio custom, ho collaborato con un dottorando, Matteo Corti, sotto la supervisione del responsabile R&D dell’azienda, Francesco Crisafi. Questo lavoro fa parte del progetto CRIMSON, il cui scopo è sviluppare un dispositivo di imaging bio-fotonico di nuova generazione basato sulla spettroscopia vibrazionale, potenzialmente rivoluzionando lo studio dell’origine cellulare delle malattie, aprendo la strada a nuovi approcci per terapie personalizzate.
Development of a custom imaging platform for broadband stimulated Raman scattering microscopy
ZOLDAN, FEDERICO
2023/2024
Abstract
This thesis project has been conducted at CRI (Cambridge Raman Imaging), a spin-off company of Politecnico di Milano and Universisty of Cambridge, whose main aim is developing a compact, coherent Raman imaging platform for in-vitro diagnostics. In particular, the start-up is specialized in stimulated Raman analysis of tissues: the idea is that the stimulated Raman spectrum can be used as a bio-marker to identify specific diseases or disorders, thus enabling the discrimination between healthy cells/tissues and unhealthy ones. This project consisted in the development, characterisation and test of a custom Stimulated Raman Scattering (SRS) microscope from scratch; this means the apparatus should be flexible, suitable for analysis of different samples (not only biological ones) and capable to encompass different optical microscopy techniques. For the realization of the system, simple optical components were adopted, without integrated devices produced by third parts. Essentiality of the setup is what makes it complex, since it is more critical. In order to build the custom microscope, I worked with a PhD student, Matteo Corti, and I was supervised by the R&D Manager of the company, Francesco Crisafi. This work belongs to CRIMSON project: CRIMSON aims to provide a next-generation bio-photonics imaging device based on vibrational spectroscopy, with the potential to revolutionize the study of the cellular origin of diseases allowing for novel approaches towards personalized therapy.File | Dimensione | Formato | |
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2024_12_Zoldan_ExecutiveSummary.pdf
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Descrizione: Executive summary Zoldan
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2024_12_Zoldan_Thesis.pdf
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Descrizione: Tesi Magistrale Zoldan
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188.5 MB
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188.5 MB | Adobe PDF | Visualizza/Apri |
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https://hdl.handle.net/10589/230172