In this thesis work the reproducibility which is possible to achieve with the electrospinning technique in the fabrication of matrices and the effects of the processing parameters on the morphology and dimension of the fibers has been investigated. Then, it was decided to study the efficiency of the Molecular Imprinting of Polymers (MIPs) on PolyActive® and, in details, on its different molecular weights and compositions (300PEOT55PBT45 and 1000PEOT70PBT30). Moreover, the adhesion and proliferation of human Mesenchymal Stem Cells (hMSCs) was investigated depending on the different electrospun polymeric materials. Finally, it was decided to analyze the answer in terms of migration of hMSCs induced from selected chemokines.
In questo lavoro di tesi è stata condotta un'indagine sulla riproducibilità ottenibile nella fabbricazione di matrici tramite la tecnica dell'elettrospinning e sugli effetti dei vari parametri di processo sulla morfologia e dimensione delle fibre stesse. Si è poi proceduto a testare l'efficienza e la selettività della tecnica del Molecular Imprinting (MIPs) su un copolimero, PoliActive(r), nei suoi diversi pesi molecolari e composizioni (300PEOT55PBT45 e 1000PEOT70PBT30). Inoltre, si è studiata l'adesione e la proliferazione di cellule staminali mesenchimali (hMSC) in risposta ai diversi materiali polimerici con cui erano stati elettrospinnati gli scaffold. Infine, è stata analizzata l'effetto delle chemochine in termini di migrazione delle hMSC.
Towards bioactive electrospun scaffolds for stem cells recruitment in regenerative medicine
SPADOLINI, GABRIELE
2010/2011
Abstract
In this thesis work the reproducibility which is possible to achieve with the electrospinning technique in the fabrication of matrices and the effects of the processing parameters on the morphology and dimension of the fibers has been investigated. Then, it was decided to study the efficiency of the Molecular Imprinting of Polymers (MIPs) on PolyActive® and, in details, on its different molecular weights and compositions (300PEOT55PBT45 and 1000PEOT70PBT30). Moreover, the adhesion and proliferation of human Mesenchymal Stem Cells (hMSCs) was investigated depending on the different electrospun polymeric materials. Finally, it was decided to analyze the answer in terms of migration of hMSCs induced from selected chemokines.File | Dimensione | Formato | |
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https://hdl.handle.net/10589/34621