In this thesis work an innovative method to obtain hybrid biofunctional scaffolds has been developed. Polyurethane foam scaffolds were coated by Layer-by-Layer technology with layers of biomolecules that are naturally present in the meniscal matrix microenvironment. These functional porous scaffolds have been mechanically (compression and tensile tests) and morphologically (SEM, MicroCT etc.) characterized proving that they match all the requirements for meniscal scaffolds. Moreover, the modified surfaces were also analyzed with immunostainings, ATR-FTIR and XPS analysis etc., and the presence of the functional coatings on PU surfaces was confirmed. Finally, h-BM MSCs were cultured on these hybrid functional surfaces and good responses were found for cell adhesion, morphology and proliferation, suggesting that the differentiation of MSCs into chondrocytes might be occurring.
In questo lavoro di tesi è stato sviluppato un metodo innovativo per l’ottenimento di scaffold ibridi e funzionalizzati. Scaffold in poliuretano sono stati rivestiti tramite tecniche Layer-by-Layer con diversi strati di biomolecole naturalmente presenti nella matrice extracellulare del menisco. Questi scaffold porosi funzionalizzati sono stati caratterizzati sia dal punto di vista mecchanico (prove a compressione e trazione) che morfologico (tramite SEM, MicroCT ecc.) dimostrando di rispettare tutti i requisiti richiesti ad uno scaffold per l’ingegneria del tessuto meniscale. Inoltre, le superfici modificate sono state analizzate anche con immunostaining, ATR-FTIR, XPS ecc., confermando la presenza dei coating funzionalizzati. Infine, risultati promettenti sono emersi dalla coltura di cellule mesenchimali staminali adulte (hBM-MSC) su tali superfici funzionali, in particolare riguardo adesione, morfologia e proliferazione cellulare, suggerendo che potrebbe essersi verificata la differenziazione delle MSC in condrociti.
Hybrid functional scaffolds for meniscal tissue engineering
CILLI, DAVIDE
2011/2012
Abstract
In this thesis work an innovative method to obtain hybrid biofunctional scaffolds has been developed. Polyurethane foam scaffolds were coated by Layer-by-Layer technology with layers of biomolecules that are naturally present in the meniscal matrix microenvironment. These functional porous scaffolds have been mechanically (compression and tensile tests) and morphologically (SEM, MicroCT etc.) characterized proving that they match all the requirements for meniscal scaffolds. Moreover, the modified surfaces were also analyzed with immunostainings, ATR-FTIR and XPS analysis etc., and the presence of the functional coatings on PU surfaces was confirmed. Finally, h-BM MSCs were cultured on these hybrid functional surfaces and good responses were found for cell adhesion, morphology and proliferation, suggesting that the differentiation of MSCs into chondrocytes might be occurring.File | Dimensione | Formato | |
---|---|---|---|
2013_04_Cilli.PDF.pdf
non accessibile
Descrizione: Testo della Tesi
Dimensione
10.26 MB
Formato
Adobe PDF
|
10.26 MB | Adobe PDF | Visualizza/Apri |
I documenti in POLITesi sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
https://hdl.handle.net/10589/77604